Step-by-step protocols for RNA interference enable consistent and reliable gene silencing experiments
Covers both classical and advanced RNAi strategies, including use of endogenous cellular machinery
Includes methods for designing siRNA, miRNA, and siRNA analogs for various biological systems
Describes applications such as immunostimulatory siRNA for therapeutic use against viral infections
Written for researchers, educators, and biotech professionals seeking practical RNAi methodologies
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Methods in Molecular Biology: Methods and Protocols Taxman, Debra J.
The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: