Step-by-step laboratory protocols enable reproducible PCR primer design experiments
Includes primer design strategies for quantitative PCR, in silico PCR, and software-based approaches for enhanced versatility
Covers troubleshooting and avoiding common pitfalls to minimize experimental errors
Authoritative and accessible content suitable for students, researchers, and professionals
Second edition with updated information and expanded troubleshooting section
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Methods in Molecular Biology Basu, Chhandak
This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, ch