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Immuno In Situ Mount™

Western blot analysis of human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blots were probed with anti-eNOS monoclonal antibody (NM2211; lanes 1 & 2), anti-eNOS (Tyr-657) phospho-specific antibody (NP4031; lanes 3 & 4), or anti-eNOS polyclonal antibody (NP2281; lanes 5 & 6).

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Anti-eNOS (Tyr-657)/nNOS (Tyr-895), Phosphospecific Antibody

Western blot analysis of Caspase expression in human Jurkat cells. The blot was probed with anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2), anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4), anti-Caspase-7 at 1:500 (lane 5) and 1:1000 (lane 6), as well as anti-Caspase-8 at 1:250 (lane 7) and 1:500 (lane 8).

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Anti-Caspase-8 (C-terminal region) Antibody

The cell morphology of MDA-MB-231 breast cancer cells after they were treated with fludioxonil(Flu) for 72 h and were stained with DAPI and actin. The samples were sealed with mounting medium (Cat. AR-6515). Image from publication, CC-BY-4.0, PMID: 39201710.

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Fluoroshield™ with DABCO

Figure 1. Oxidation of Guanosine

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DNA Damage ELISA Kit

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Zymo-Spin IICR Columns

Western blot of human recombinant PD-L1 extracellular region (40 kDa; lanes 1 & 3) and endogenous PD-L1 in human lung (lanes 2 & 4). The blot was probed with mouse monoclonals anti-PD-L1 (PM0511) at 1:500 (lanes 1 & 2) and anti-PD-L1 (PM0801) at 1:250 (lanes 3 & 4).

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Anti-PD-L1 (Extracellular region) M080 Antibody

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Glycerol mounting medium with DABCO

Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) TP5691 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).

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Anti-TRPM7 (cytoplasmic region) Antibody

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ImmunoFluoroMount with PPD

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Zymoclean Gel DNA Recovery Kit

Western blot image of human A431 (lane 1), HepG2 (lane 2), PC3 (lane 3), Jurkat (lane 4), bovine tubulin (lane 5), and recombinant human ALDH1A1 (lane 6). The blot was probed with mouse monoclonal ALDH1A1 M562 (lanes 1-6) at a dilution of 1:1,000.

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Anti-ALDH1A1 (N-terminal region) Antibody

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Ad Empty Adenovirus

Western blot analysis of human full length MuRF1 recombinant protein. The blot was probed with mouse monoclonal MuRF1 (C-terminal region) at 1:250 (lane 1) and 1:1000 (lane 2) and rabbit polyclonal MuRF1 (C-terminal region) at 1:1000 (lanes 3) and 1:4000 (lane 4).

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Anti-MuRF1 (C-terminal region) Antibody

Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B (C-terminus) (lanes 1 & 2), anti-Histone H2B (Ser-36) (lanes 3 & 4), and anti-Histone H2B (a.a. 33-47) (lanes 5 & 6).

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Anti-Histone H2B (C-terminus) Antibody

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Fluoroshield with Phenylenediamine

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Fluoroshield with DAPI & PPD

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Immuno Fluoro Mount with DABCO and DAPI

Figure 1. Dilute samples within the detection limit of the ELISA

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General Serum Diluent

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Bovine A1 Beta casein (A1) ELISA Assay

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Human NGFR/p75ECD Rapid ELISA Assay

Western blot image of TRPM7 expression in human MCF-7 cell lysate. The blot was probed with mouse monoclonal anti-TRPM7 (Extracellular region) M573 at a dilution of 1:250.

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Anti-TRPM7 (Extracellular region) Antibody

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Ad CMV Luciferase Adenovirus

Western blot image of human recombinant Atrogin 1 (lanes 1-6). The blot was probed with rabbit polyclonal Atrogin-1 (lanes 1-3) and rat monoclonal Atrogin-1 (lanes 4-6) at 1:1000 (lanes 1 & 4), 1:2000 (lanes 2 & 5), and 1:4000 (lanes 3 & 6).

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Anti-Atrogin-1 Antibody

Western blot analysis of mDia3 expression in human Jurkat cells treated with Calyculin A (100 nM) (lanes 1-4). The blots were treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal anti-mDia3 (C-terminus; DP4511) (lanes 1 & 2) and anti-phospho-mDia3 (Ser-196; DP4521) (lanes 3 & 4).

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Anti-mDia3 (Ser-196)) [conserved site], Phosphospecific Antibody

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Zymo-Spin II Columns

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Eosin Y

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Ad CMV hTSHR 289 Adenovirus

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Anti-Neuronal nuclei antigen [NeuN]/FOX3, (NeuN/FOX3) Antibody

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit phosphorylated at Tyr1336 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

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Anti-NMDA NR2B Subunit (Tyr1336) Antibody

Genovis Inc.

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Anti-Glutathione peroxidase 3 (GPx-3) Antibody

Distribution of α2δ-1 subunit in spinal dorsal horn. The spinal dorsal horn sections of CV mice were stained with anti-α2δ-1 antibody (green). Scale bar = 200 µm. Solid line represents the gray matter border, dotted line represents the superficial layer. Sections mounted with Fluoroshield medium (Cat. AR-6500, ImmunoBioScience Corp). Image from publication CC-BY-4.0. PMID: 38989140

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Fluoroshield™

Western blot analysis of mouse heart tissue (lanes 1 & 3) or C2C12 cells (lanes 2 & 4). The blot was probed with anti-MuRF1 (C-terminal region) (lanes 1 & 2) or anti-Atrogin-1 (lanes 3 & 4).

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Anti-MuRF1 (C-terminal region) Antibody

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Anti-Superoxide dismutase 1 (SOD1) Antibody

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Normal Sheep IgG from non-immunized animal, Sheep Polyclonal Antibody

Immunofluoresence of FLAG-tagged AIF1 transfected COS cells showing AIF1 staining in red (Cat. 91-AIF1, 1:50) and FLAG staining in green causing positive transfected cells to appear yellow. DNA is stained blue with DAPI.

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Anti-AIF1/Iba1 Antibody

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Ad 35 Wild-Type Adenovirus Particle

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HRP Goat Anti-Human IgG Fc

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Organo / Limonene Mount™

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DNA Clean & Concentrator-5

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Hematoxylin, Immuno/Histo Aqueous

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Ad 4 Wild-Type Adenovirus Particle

Western blot analysis of connexin-43 expression in adult mouse brain (lanes 1-3). The blots were probed with mouse monoclonal anti-connexin-43 at 1:250 (lane 1), 1:500 (lane 2), and 1:1000 (lane 3).

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Anti-Connexin-43 (C-terminal region) Antibody

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Anti-Leu-Enkephalin Antibody

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Immuno Fluoro Mount with DABCO™

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Avidin/Biotin Blocking Kit Set

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Immuno Fluoro Mount™

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing cells stained by anti-NeuN (Rbfox3) antibody. The sections were stained with Aves Labs anti-NeuN polyclonal antibody (visualized in green, Cat No. NeuN-1010). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

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Anti-Neu-N (Fox 3) Antibody

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Anti-Superoxide dismutase 1 (SOD1) Antibody

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Human mBDNF CE-Marked ELISA Assay

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ELISA Plate Sealing Covers

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Anti-NGFR/p75 neurotrophin receptor (p75NTR) Antibody

Western blot of adult mouse brain lysate. The blot was probed with mouse monoclonal anti-Syntaphilin (N-terminal region) antibody at 1:250 (lane 1), 1:1000 (lane 2), or 1:2000 (lane 3).

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Anti-Syntaphilin (N-terminal region) Antibody

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Zymo-Spin IIC-XLR Columns

Figure 1. Quantification of cell death

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Cell-mediated Cytotoxicity Assay: Basic Cytotoxicity Test

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Ad CMV CD54 Adenovirus

Western blot of Drosophila lysate showing specific labeling of the ~68 kDa AKT protein phosphorylated at Thr342 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

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Anti-AKT (Thr342) Antibody

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Anti-Nicastrin, Central region Antibody

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ImmunoHistoMount™

Immunocytochemical labeling of Trop-2 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Trop-2 (clone M005). The antibody was detected using goat anti-mouse DyLight® 594.

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Anti-Trop-2/TACSTD2 (Extracellular region) Antibody

Figure 1. Jurkat cells were stained with DAF-2DA dye (Kit 9155), washed, and then treated with DMSO control (left histogram) or 1 mM DEA NONOate, a nitric oxide donor (middle histogram). Cells were read on the FL1 channel of a flow cytometer. The median fluorescence intensity (MFI) of DEA NONOate treated cells was 3.5-fold higher than for the control cells. Data also overlaid in a single plot (right, black: Negative; right, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 227:76.

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Nitric Oxide Synthase Assay

Western blot image of human endothelial cells untreated or treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-N-Cadherin (Cytoplasmic), anti-N-Cadherin (Tyr-820), anti-N-Cadherin (a.a. 853-864), and anti-N-Cadherin (Tyr-860).

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Anti-N-Cadherin (Y860) [E-Cadherin (Y835)], Phosphospecific Antibody

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Anti-Neuron specific enolase (NSE) Antibody

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ZymoBIOMICS MagWash 1

Western blot analysis of Memo expression in adult mouse heart (lane 1 & 4), mouse C2C12 cells (lane 2 & 5), and rabbit spleen fibroblast cells (lane 3 & 6). The blot was probed with anti-Memo (N-terminal region) (MP3721; lanes 1-6) in the presence (lanes 4-6) or absence (lanes 1-3) of Memo blocking peptide (MX3725).

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Anti-Memo (N-terminal region) Antibody

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Ad CMV mouse TGFb Adenovirus

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Anti-Ionized Calcium Binding Adapter Molecule 1 (IBA1) Antibody

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Anti-Growth Associated Protein 43 (GAP-43) Antibody

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Zymo-Spin IC Columns

Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals EM5041 anti-EB1 (lane 1), EM5061 anti-EB1/2/3 (lane 2), EM5081 anti-EB2 (lane 3), EM5101 anti-EB3 (lane 4), and EM5091 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific:HRP (RS3121).

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Anti-EB2 Antibody

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Ad CMV IL-18 Adenovirus

Immunofluorescence of a section of rat brain stem showing specific labeling of MAP2 (Cat.1099-MAP2, 1:2000, red) in the perikarya and dendrites of neurons and specific labeling of the myelin sheath around axons with anti-MBP (Cat.1120-MBP , 1:5000, green). The blue is DAPI revealing nuclear DNA.

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Anti-Microtubule Associated Protein 2 (MAP2) Antibody

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Select-a-Size DNA Clean & Concentrator Kit

Immunofluorescent staining of HeLa cells using 2 µg/mL Aves Labs chicken anti-Giantin (GOLGB1) (Cat. GOLGB1-0100) antibody (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). Anti-Giantin specifically stains the Golgi apparatus in HeLa cells.

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Fluoroshield™ with DAPI

Representative section of formalin fixed, paraffin-embedded rat brain hippocampus region showing staining of GFAP. The sections were stained with Antibodies Incorporated anti-GFAP antibody (visualized in green) (Cat. 75-240) at 1:500 dilution. The stained sections were mounted with Antibodies Incorporated FluoroshieldTM with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain.

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Fluoroshield with DAPI & DABCO

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Fluoroshield with DAPI & Propylgallate

Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.

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Anti-Phosphoserine/threonine Antibody

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Genomic DNA Clean & Concentrator-10

Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) TP5651 (lanes 1 & 2) or anti-TRPM7 (Ser-1569), phospho-specific (lanes 3 & 4).

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Anti-TRPM7 (Ser-1569), Phosphospecific Antibody

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Glycerol mounting medium with DAPI and DABCO

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Ad CMV Cyto LacZ Adenovirus

Western blot of native lysates including human EpCAM extracellular region (lane 1 & 6), MCF7 breast carcinoma (lane 2 & 7), A431 skin adenocarcinoma (lane 3 & 8), NCI-H1915 lung carcinoma (lane 4 & 9), and NCI-H446 lung carcinoma (lane 5 & 10). The blot was probed with mouse monoclonal anti-EpCAM (EM0391) (lanes 1-5) and mouse monoclonal anti-EpCAM (EM0421) (lanes 6-10) at 1:1000 each.

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Anti-EpCAM (Extracellular region) Antibody

Western blots of human recombinant Sema3A/Fc chimera (95/125 kDa) (lanes 1-4). The blots were probed with rabbit polyclonal Sema3A (Central region) at 1:250 (lane 1) and 1:1000 (lane 2) and mouse monoclonal Sema3A (Central region) at 1:250 (lane 3) and 1:1000 (lane 4). Both antibodies recognize the 95 kDa and 125 kDa forms of the recombinant Sema3A.

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Anti-Semaphorin-3A (Central region) Antibody

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ZR 1 kb DNA Marker

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One step Polymer HRPMouse and Rat IgG with DAB

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Ad CMV p53 Adenovirus

Western blot of mouse testes lysate showing specific immunolabeling of the ~46 kDa eukaryotic initiation factor 4A2, eIF4A2, protein.

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Anti-eIF4A2 Antibody

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Zymo-Spin IIICG Columns