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Immunofluorescent staining of HeLa cells using 2 µg/mL Aves Labs chicken anti-Giantin (GOLGB1) (Cat. GOLGB1-0100) antibody (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. The cells were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). Anti-Giantin specifically stains the Golgi apparatus in HeLa cells.

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Western blot image of mouse recombinant NLRC4 (Ser-533) phosphorylated peptide (lanes 1 & 4) and dephosphorylated peptide (lanes 2 & 5), as well as human PMA-differentiated THP1 cells (lanes 3 & 6). The blots were probed with rabbit polyclonals anti-NLRC4 (a.a. 525-538) (lanes 1-3) and anti-NLRC4 (Ser-533) phospho-specific (lanes 4-6).

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Immunofluorescence of a section of rat hippocampus selectively labeling the neuronal cilia with anti-adenylate cyclase III (Cat. 85-AC3, 1:10,000, red), labeling nuclei with anti-MECP2 (Cat. 1205-MeCP2, 1:1000,green), and Hoechst staining of nuclear DNA.

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Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).

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Immunofluorescence of serum starved, FBS stimulated HeLa cells showing nuclear labeling of activated cells with Anti- c-FOS (Cat. 309-cFOS, 1:1000, green) while Anti-Vimentin (Cat. 2105-VIM, 1:500, red) labels the cytoplasmic intermediate filament, and nuclear staining with DAPI (blue).

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Western blot of FHOD1 phosphorylation in human K562 cells stimulated with calyculin A (100 nM) for 30 min. (lanes 1 & 3). The blot was then treated with lambda phosphatase (lanes 2 & 4). Blots were probed with mouse polyclonal anti-FHOD1 (lanes 1 & 2) and anti-FHOD1 (Thr-1141), phospho-specific antibody (lanes 3 & 4) .

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Immunofluorescence of a section of rat brain stem showing specific labeling of MAP2 (Cat.1099-MAP2, 1:2000, red) in the perikarya and dendrites of neurons and specific labeling of the myelin sheath around axons with anti-MBP (Cat.1120-MBP , 1:5000, green). The blue is DAPI revealing nuclear DNA.

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Immunofluorescent staining of MCF7 cells using 1 µg/mL chicken anti-HB9 (MNX1) antibody (green). Actin filaments were stained using Phalloidin (red). The cells were mounted with ICT's Fluoroshield mounting medium (Cat. AR-6500). Anti-HB9/MNX1 specifically stains the nucleus of MCF7 cells.

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Anti-EphA4 (N-terminal region) Antibody

Immunolabeling of HeLa cells showing vesicular staining of LAMP1 protein (Cat. 1030-LAMP1, red, 1:2000) accumulated in swollen lysosomes, while Anti-Vimentin (Cat. 2105-VIM, green, 1:500) specifically labels the intermediate filament network in these cells. The blue stain is DAPI to reveal nuclear DNA.

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Western blot analysis of human full length MuRF1 recombinant protein. The blot was probed with mouse monoclonal MuRF1 (C-terminal region) at 1:250 (lane 1) and 1:1000 (lane 2) and rabbit polyclonal MuRF1 (C-terminal region) at 1:1000 (lanes 3) and 1:4000 (lane 4).

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Anti-MuRF1 (C-terminal region) Antibody